This density can be realized by hexagonal layers (in continuum many ways). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.Ĭonflict of interest-The authors declare that they have no conflicts of interest with the contents of this article.Finding the densest (not necessarily periodic) ball packings in 3-dimensional Euclidean space is known as the Kepler Conjecture: No packing of spheres of equal radius has a density greater than the face-centred cubic packing. M.) and by NIGMS, National Institutes of Health, Grants R35 GM127085 (to T.
project administration.įunding and additional information-This work was supported by NCI and NIGMS, National Institutes of Health, Grants R01 CA188347, P30CA056036, and R01 GM136717 and by a Drexel-Coulter Program Award (to A. This article contains supporting information.Īuthor contributions-O. That depends on RPA-mediated DNA replication restart. Role of this protein in RNA metabolism and suggesting a mechanism of genome maintenance These results demonstrate that RPA binds RNA with high affinity, supporting the In reconstitution experiments, we showed that human DNA polymerasesĬan utilize RPA-generated R-loops for initiation of DNA synthesis, mimicking the process Furthermore, RPA, by forming a complex with RNA, can promote R-loop formation with Using gel-retardation and RNA/DNA competitionĪssays, we found that RPA binds RNA with an unexpectedly high affinity ( KD ≈ 100 p m). Here, we investigated the RNA-binding properties of human RPA and However, the mechanism of R-loop formation Recombination and DNA replication restart. Including humans, and may play an important role in transcription-coupled homologous R-loops are common in the genomes of pro- and eukaryotes, RPA stimulates RNA-templated DNA repair in vitro and associates in vivo with R-loops, the three-stranded structures consisting of an RNA-DNA hybrid and theĭisplaced ssDNA strand. However, recent data suggest that RPA may It was presumed that RPA binds RNA weakly. To perform its functions, RPA binds ssDNA tightly. Replication protein A (RPA), a major eukaryotic ssDNA-binding protein, is essentialįor all metabolic processes that involve ssDNA, including DNA replication, repair,Īnd damage signaling. Glycobiology and Extracellular Matrices.
0 kommentar(er)
